Development and Characterization of Two Types of Surface Displayed Levansucrases for Levan Biosynthesis

Levan has wide applications in chemical, cosmetic, pharmaceutical and food industries. The free levansucrase is usually used the biosynthesis of levan, but poor reusability low stability have limited its large-scale use. To address this problem, surface-displayed Saccharomyces cerevisiae were generated evaluated study. from Zymomonas mobilis was displayed on cell surface EBY100 using a various yeast display platform. N-terminal fusion partner based a-agglutinin, C-terminal one Flo1p. yield levan produced by these two whole-cell biocatalysts reaches 26 g/L 34 24 h, respectively. Meanwhile, levansucrases significantly enhanced. After six reuses, retained over 50% 60% their initial activities, Furthermore, molecular weight polydispersity test products suggested that biocatalyst Flo1p more potentials production with which critical certain applications. In conclusion, our method not only enable possibility to reuse enzyme, also improves enzyme.